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25112 Heterophile Blocking Reagent

Heterophile Blocking Reagent
Test Code: HBR
Synonyms/Keywords
HBR, Scantibodies, Heterophile Antibodies​
Test Components
The analyte of interest must be commented at the time of the Heterophile Blocking Reagent (HBR) request (assay for which interference is suspected); example comment "TSH for HBR studies". ​
Useful For
The Heterophile Blocking Reagent is used as a method of detecting the presence of heterophile antibodies in a patient's serum or plasma. Samples for a particular analyte are analyzed untreated, then again after treatment with the HBR reagents. If a significant change in analyte concentration is observed versus the original untreated value, then heterophile antibody interference is suspected. ​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum/ Plasma​ Red Top (RTT)​ Lithium-heparin Plasma Separator (PST)
Lithium or Sodium-heparin Green Top (GTT)​		 1 mL​ 0.5 mL​ ​
Collection Processing Instructions
The analyte of interest must be commented at the time of the Heterophile Blocking Reagent (HBR) request (assay for which interference is suspected); example comment "TSH for HBR studies".
 
The HBT is for antigen assays only. For antibody detection assays, a non-specific antibody blocking tube should be used.
 
NOTE: Requests for HBR testing are limited to Endocrinologists with approved access to this test. Any other request must be approved through Interpretive Contact or Technical Manager listed.​
Specimen Stability Information
Specimen Type Temperature Time
Same criteria as assay to be investigated. ​ ​ ​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ Less than 2 hours​ Scantibodies Heterophile Blocking Tube Reagent  ​
Test Information
A heterophilic sample is a serum/plasma sample that contains non-specific antibodies which are able to bind to animal antibodies used in some immunoassay test systems. The most common interference effect is a false positive assay result, although false negatives have also been reported. If a physician suspects a test result is falsely increased (or decreased), he/she may request the assay be repeated after treatment with Heterophile Blocking Reagent.
 
The Heterophile Blocking Reagent is used as a method of detecting the presence of heterophile antibodies in a patient's serum or plasma. Samples for a particular analyte are analyzed untreated, then again after treatment with the HBR reagents. If a significant change in analyte concentration is observed versus the original untreated value, then heterophile antibody interference is suspected. ​
Reference Range Information
Performing Location Reference Range
Marshfield​ See patient report comments for interpretation of results​
Interpretation
An interpretation of the HBR study results will be commented under the appropriate result code. In addition, the following comment will be appended to each HBR assay reported: "Results obtained with (Scantibodies) blocking reagents are an estimate, should be used as an adjunct to other data (symptoms, other test results, clinical impressions, etc.), and may not reflect the exact value."
 
Interpretation only available Monday-Friday 8am-5pm.​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
Synonyms/Keywords
HBR, Scantibodies, Heterophile Antibodies​
Test Components
The analyte of interest must be commented at the time of the Heterophile Blocking Reagent (HBR) request (assay for which interference is suspected); example comment "TSH for HBR studies". ​
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum/ Plasma​ Red Top (RTT)​ Lithium-heparin Plasma Separator (PST)
Lithium or Sodium-heparin Green Top (GTT)​		 1 mL​ 0.5 mL​ ​
Collection Processing
The analyte of interest must be commented at the time of the Heterophile Blocking Reagent (HBR) request (assay for which interference is suspected); example comment "TSH for HBR studies".
 
The HBT is for antigen assays only. For antibody detection assays, a non-specific antibody blocking tube should be used.
 
NOTE: Requests for HBR testing are limited to Endocrinologists with approved access to this test. Any other request must be approved through Interpretive Contact or Technical Manager listed.​
Specimen Stability Information
Specimen Type Temperature Time
Same criteria as assay to be investigated. ​ ​ ​
Useful For
The Heterophile Blocking Reagent is used as a method of detecting the presence of heterophile antibodies in a patient's serum or plasma. Samples for a particular analyte are analyzed untreated, then again after treatment with the HBR reagents. If a significant change in analyte concentration is observed versus the original untreated value, then heterophile antibody interference is suspected. ​
Test Components
The analyte of interest must be commented at the time of the Heterophile Blocking Reagent (HBR) request (assay for which interference is suspected); example comment "TSH for HBR studies". ​
Reference Range Information
Performing Location Reference Range
Marshfield​ See patient report comments for interpretation of results​
Interpretation
An interpretation of the HBR study results will be commented under the appropriate result code. In addition, the following comment will be appended to each HBR assay reported: "Results obtained with (Scantibodies) blocking reagents are an estimate, should be used as an adjunct to other data (symptoms, other test results, clinical impressions, etc.), and may not reflect the exact value."
 
Interpretation only available Monday-Friday 8am-5pm.​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Sunday​ Less than 2 hours​ Scantibodies Heterophile Blocking Tube Reagent  ​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
For most current information refer to the Marshfield Laboratory online reference manual.