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25227 Lyme Immunoblot, IgM

Lyme Immunoblot, IgM
Test Code: LYIBM
Synonyms/Keywords
​Lyme Immunoblot, IgM, Lyme Western Blot, IgM (LYBL-M)
Useful For
​Confirmation of Lyme serology EIA positive and equivocal results.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​1.0 mL ​0.5 mL ​0.5 mL
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​Refrigerate ​5 days
​ ​Freeze ​> 5 days
Rejection Criteria
Hemolyzed, lipemic, bilirubinemic, or turbid sera
​CSF
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday, Wednesday, Friday (summer) ​1 day ​Immunoblot
​Marshfield ​Fridays (off season) ​1 day ​Immunoblot
Test Information
According to the Centers for Disease Control and Prevention, during the first month of acute illness, all positive and equivocal screening test results should be confirmed by IgM and IgG immunoblot or by IgG immunoblot alone.  After the first month of acute illness, all positive and equivocal screening test results should be confirmed by IgG immunoblot alone.
 
Immunoblots should not be used as the primary test for the serological diagnosis of Lyme disease as they are no more sensitive than Lyme EIA assays.
 
Reference Range Information
Performing Location Reference Range
Marshfield​ ​Negative
Interpretation
​The band pattern of this blot has been interpreted using CDC criteria.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86617 ​ ​ ​ ​
Synonyms/Keywords
​Lyme Immunoblot, IgM, Lyme Western Blot, IgM (LYBL-M)
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ ​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​1.0 mL ​0.5 mL ​0.5 mL
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​Refrigerate ​5 days
​ ​Freeze ​> 5 days
Rejection Criteria
Hemolyzed, lipemic, bilirubinemic, or turbid sera
​CSF
Useful For
​Confirmation of Lyme serology EIA positive and equivocal results.
Reference Range Information
Performing Location Reference Range
Marshfield​ ​Negative
Interpretation
​The band pattern of this blot has been interpreted using CDC criteria.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday, Wednesday, Friday (summer) ​1 day ​Immunoblot
​Marshfield ​Fridays (off season) ​1 day ​Immunoblot
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86617 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.