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25483 OB/ER Only HIV 1/2 Rapid Test

OB/ER Only HIV 1/2 Rapid Test
Test Code: RPHIV
Synonyms/Keywords
​​​​HIV
Useful For
Identifying HIV infection in O.B. and E.R. patients with unknown HIV serostatus at time of delivery and at time of presentation in E.R.
Restricted Use: This test is only orderable and valid for Marshfield Medical Center (01), St. Clare's Hospital (71), and Lakeview Medical Center (64).
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
No​ Plasma ​EDTA Pink Top Tube (PTT) ​EDTA Lavender Top Tube (LTT) ​1 mL ​0.5 mL ​
Collection Processing Instructions
      ·    Centrifugation and refrigerated/frozen storage must occur within 24 hours of collection for either  
            sample type.
·      EDTA plasma samples may be centrifuged immediately after collection.
·      If serum is inadvertently collected (see note below), complete clot formation should take place before centrifugation.
·      After centrifugation, physically separate plasma/serum from the red cells and send aliquot for analysis.
·       Specimens must be free of particulate matter including fibrin.
 
            Note: Serum can only be used for initial HIV testing, it cannot be used for Mayo HVDIP confirmation
            testing of REACTIVE samples.
 
·       Specimens are stable for 24 hours at room temperature, this includes pre- & post- centrifugation time, separated or unseparated from red cells.
·       Store plasma/serum specimens at 2–8°C if not tested within 24 hours of collection.  Separated specimens are stable for 14 days at 2–8ºC.
·       For longer storage, plasma/serum may be frozen for 8 months at -20ºC or colder. Samples may undergo up to 5 freeze/thaw cycles.
·       Frozen samples must be completely thawed, thoroughly mixed, and centrifuged before analysis.
·       For U-have testing, specimens are stable in primary tubes up to 14 days at 2–8°C (this includes plasma stored on packed red cells, serum stored on the clot, and specimens processed and stored in gel barrier blood collection tubes).
 
            If REACTIVE, Mayo Medical Labs will require an additional 1 mL of EDTA plasma.
Specimen Stability Information
Specimen Type Temperature Ti​​me
Plasma ​Ambient ​24 hours
​Refrigerate ​14 days
​Frozen (-20 oC)  ​8 months
Rejection Criteria
​Grossly icteric specimens are uninterpretable due to high background
​Specimens containing precipitate
​Cadaver specimens
​Heat-inactivated specimens
​Body fluids other than serum
Samples stored at room temperature for longer than 24 hours,
this includes pre- and post- centrifugation time.
Samples stored for longer than 14 days at 2–8°C.
​Specimens with obvious microbial contamination.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrum​​entation
Diagnostic & Treatment Center​ ​Monday through Sunday ​2 hours ​Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
​Eau Claire ​Monday through Sunday 2 hours ​Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
​Lakeview Medical Center ​Monday through Sunday ​2 hours ​​Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
​Marshfield ​Monday through Sunday ​2 hours ​Sandwich Chemiluminometric IA/Siemens Centaur
​Minocqua​Monday through Sunday​2 hours​Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
Test Information
This test is for use by OB and ER providers only, for patients presenting that have no known HIV status, and the risk of exposure to blood is significant during the patient encounter. Do not order this test for the routine determination of HIV serostatus: Use HIV-1,2 Antibody EIA with Confirm Eval. Do not order for identifying HIV infection in source patient in a known exposure incident with a healthcare worker: Use HIV-1/2 Exposure Screen.
Reference Range Information
Performing Location Reference​​ Range
All sites​ ​Non-Reactive
Interpretation
Western blot analysis is no longer recommended for confirmation of HIV EIA antibody-positive results. Confirmation of rapid HIV 1,2 antibody testing will be performed at Mayo Medical Labs. This test algorithm closely follows the current CDC recommendations for HIV diagnosis. (See Centers for Disease Control and Prevention. Detection of Acute HIV Infection in Two Evaluations of a New HIV Diagnostic Testing Algorithm — United States, 2011–2013. MMWR 2013;62:489-494.)
Outreach CPTs
​
CPT Modifier
(if needed)		 Quantity Description Comments
87389 ​ ​ ​1 ​HIV-1 Ag(s), with HIV-1 and HIV-2 Abs single result ​For testing performed at Marshfield
​86703 ​92 ​1 ​HIV-1 and HIV-2 Abs single result ​For testing performed at Regional Center Labs
Synonyms/Keywords
​​​​HIV
Ordering Applications
Ordering Application Description​​
​Clinical Order Manager ​OB/ER Only HIV 1/2 Rapid Test
​Centricity ​OB/ER Only HIV 1/2 Rapid Test
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)​​
No​ Plasma ​EDTA Pink Top Tube (PTT) ​EDTA Lavender Top Tube (LTT) ​1 mL ​0.5 mL ​
Collection Processing
      ·    Centrifugation and refrigerated/frozen storage must occur within 24 hours of collection for either  
            sample type.
·      EDTA plasma samples may be centrifuged immediately after collection.
·      If serum is inadvertently collected (see note below), complete clot formation should take place before centrifugation.
·      After centrifugation, physically separate plasma/serum from the red cells and send aliquot for analysis.
·       Specimens must be free of particulate matter including fibrin.
 
            Note: Serum can only be used for initial HIV testing, it cannot be used for Mayo HVDIP confirmation
            testing of REACTIVE samples.
 
·       Specimens are stable for 24 hours at room temperature, this includes pre- & post- centrifugation time, separated or unseparated from red cells.
·       Store plasma/serum specimens at 2–8°C if not tested within 24 hours of collection.  Separated specimens are stable for 14 days at 2–8ºC.
·       For longer storage, plasma/serum may be frozen for 8 months at -20ºC or colder. Samples may undergo up to 5 freeze/thaw cycles.
·       Frozen samples must be completely thawed, thoroughly mixed, and centrifuged before analysis.
·       For U-have testing, specimens are stable in primary tubes up to 14 days at 2–8°C (this includes plasma stored on packed red cells, serum stored on the clot, and specimens processed and stored in gel barrier blood collection tubes).
 
            If REACTIVE, Mayo Medical Labs will require an additional 1 mL of EDTA plasma.
Specimen Stability Information
Specimen Type Temperature Ti​​me
Plasma ​Ambient ​24 hours
​Refrigerate ​14 days
​Frozen (-20 oC)  ​8 months
Rejection Criteria
​Grossly icteric specimens are uninterpretable due to high background
​Specimens containing precipitate
​Cadaver specimens
​Heat-inactivated specimens
​Body fluids other than serum
Samples stored at room temperature for longer than 24 hours,
this includes pre- and post- centrifugation time.
Samples stored for longer than 14 days at 2–8°C.
​Specimens with obvious microbial contamination.
Useful For
Identifying HIV infection in O.B. and E.R. patients with unknown HIV serostatus at time of delivery and at time of presentation in E.R.
Restricted Use: This test is only orderable and valid for Marshfield Medical Center (01), St. Clare's Hospital (71), and Lakeview Medical Center (64).
Reference Range Information
Performing Location Reference​​ Range
All sites​ ​Non-Reactive
Interpretation
Western blot analysis is no longer recommended for confirmation of HIV EIA antibody-positive results. Confirmation of rapid HIV 1,2 antibody testing will be performed at Mayo Medical Labs. This test algorithm closely follows the current CDC recommendations for HIV diagnosis. (See Centers for Disease Control and Prevention. Detection of Acute HIV Infection in Two Evaluations of a New HIV Diagnostic Testing Algorithm — United States, 2011–2013. MMWR 2013;62:489-494.)
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrum​​entation
Diagnostic & Treatment Center​ ​Monday through Sunday ​2 hours ​Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
​Eau Claire ​Monday through Sunday 2 hours ​Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
​Lakeview Medical Center ​Monday through Sunday ​2 hours ​​Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
​Marshfield ​Monday through Sunday ​2 hours ​Sandwich Chemiluminometric IA/Siemens Centaur
​Minocqua​Monday through Sunday​2 hours​Lateral flow immunoassay/Trinity Biotech Uni-Gold Recombigen HIV-1/2 Rapid Test
For billing questions, see Contacts
Outreach CPTs
​
CPT Modifier
(if needed)		 Quantity Description Comments
87389 ​ ​ ​1 ​HIV-1 Ag(s), with HIV-1 and HIV-2 Abs single result ​For testing performed at Marshfield
​86703 ​92 ​1 ​HIV-1 and HIV-2 Abs single result ​For testing performed at Regional Center Labs
For most current information refer to the Marshfield Laboratory online reference manual.