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25508 Protein-Immunotyping (PEP,GAM,IT) Panel

Protein-Immunotyping (PEP,GAM,IT) Panel
Test Code: IEPIT
Synonyms/Keywords
​IF Panel (if Immunotyping is required by interpreter), IEP, Immunoelectrophoresis panel - includes protein electrophoresis, immunoglobulins IgG, IgA and IgM and ​Immunotyping if needed). 
IT Panel
Test Components
​Protein Electrophoresis (Total Protein, Albumin, Alpha-1, Alpha-2, Beta, and Gamma Globulins), Immunoglobulin IgG, IgA and IgM.  Immunotyping, if required by interpreter (there is an additional charge for Immunotyping).
Useful For
Protein electrophoresis is a useful tool in screening or monitoring patients with protein abnormalities such as hypoproteinemia, hepatodegenerative disease, acute and chronic phase reactions, anemia, polyclonal and monoclonal gammopathies and dysproteinemias.  Quantitative immunoglobulins are useful in detecting or monitoring of monoclonal gammopathies or immune deficiencies.  Immunotyping is useful in determining or ruling out a monoclonal gammopathy.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT)​ ​Serum Separator Tube (SST) 2 mL ​ 1 mL ​ 1 mL
Collection Processing Instructions

Separate serum/plasma from cells within 2 hours of collection.
Please make 3 aliquots of serum for this test panel.​

Specimen Stability Information
Specimen Type Temperature Time
​Serum​ ​
​
 Refrigerate​ (Preferred)
 10 days​
​Frozen (only 1 freeze/thaw cycle)
​30 days
Rejection Criteria

​Plasma

Performing Laboratory Information
 
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​

6-8 hours

If Immunotyping is required - an additional 24 hours should be expected​

Protein Electrophoresis – Capillary electrophoresis / Sebia Capillarys 2 protein system​

Immunoglobulins – Turbidimetric method / The Binding Site, Optilite analyzer

Immunotyping electrophoresis/Sebia Capillarys 2 Protein System​

Total serum protein: Biuret Blanked Endpoint Assay/Beckman AU 

Test Information
Includes total serum protein, serum protein electrophoresis by Capillary Electrophoresis and quantitative immunoglobulins (IgG, IgA and IgM).  Phenotyping of heavy chains and light chains (kappa and lambda) may also be performed by Immunotyping if required for pathologist/PhD interpretation and will be charged separately. ​
Reference Range Information
Performing Location Reference Range
Marshfield​

Albumin:  3.70-4.90 g/dL

Alpha-1 globulin: 0.20-0.40 g/dL

Alpha-2 globulin: 0.50-0.90 g/dL

Beta-globulin: 0.60- 1.00 g/dL

Gamma-globulin: 0.60-1.40 g/dL

 

Refer to link provided for Protein, Total Reference Ranges: 

Protein, Total

Refer to link provided for Immunoglobulin Reference Ranges:

Immunoglobulin Reference Ranges

Interpretation
Increased serum immunoglobulin concentrations occur due to polyclonal or oligoclonal immunoglobulin proliferation in hepatic disease (hepatitis, liver cirrhosis), connective tissue diseases, acute and chronic infections, as well as in the cord blood of neonates with intra uterine and perinatal infections. Elevations of IgG, IgA, or IgM may occur in monoclonal gammopathies such as multiple myeloma (IgG, IgA), macroglobulinemia (IgM), primary systemic amyloidosis, monoclonal gammopathy of undetermined significance (MGUS), and related disorders.
Decreased levels are found in patients with primary or secondary immune deficiencies.​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
84165​ ​ ​ Protein electrophoresis​ ​
82784​ ​ 3​ Quantitative immunoglobulins (IgG, IgA, IgM)​ ​
86334​ ​ ​ Immunotyping (if required)​ ​

​84155
​​​Total serum protein​
​
Synonyms/Keywords
​IF Panel (if Immunotyping is required by interpreter), IEP, Immunoelectrophoresis panel - includes protein electrophoresis, immunoglobulins IgG, IgA and IgM and ​Immunotyping if needed). 
IT Panel
Test Components
​Protein Electrophoresis (Total Protein, Albumin, Alpha-1, Alpha-2, Beta, and Gamma Globulins), Immunoglobulin IgG, IgA and IgM.  Immunotyping, if required by interpreter (there is an additional charge for Immunotyping).
Ordering Applications
Ordering Application Description
​Cerner ​Protein-Immunotyping (PEP, GAM, IT) Panel​

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Serum​ Red Top Tube (RTT)​ ​Serum Separator Tube (SST) 2 mL ​ 1 mL ​ 1 mL
Collection Processing

Separate serum/plasma from cells within 2 hours of collection.
Please make 3 aliquots of serum for this test panel.​

Specimen Stability Information
Specimen Type Temperature Time
​Serum​ ​
​
 Refrigerate​ (Preferred)
 10 days​
​Frozen (only 1 freeze/thaw cycle)
​30 days
Rejection Criteria

​Plasma

Useful For
Protein electrophoresis is a useful tool in screening or monitoring patients with protein abnormalities such as hypoproteinemia, hepatodegenerative disease, acute and chronic phase reactions, anemia, polyclonal and monoclonal gammopathies and dysproteinemias.  Quantitative immunoglobulins are useful in detecting or monitoring of monoclonal gammopathies or immune deficiencies.  Immunotyping is useful in determining or ruling out a monoclonal gammopathy.​
Test Components
​Protein Electrophoresis (Total Protein, Albumin, Alpha-1, Alpha-2, Beta, and Gamma Globulins), Immunoglobulin IgG, IgA and IgM.  Immunotyping, if required by interpreter (there is an additional charge for Immunotyping).
Reference Range Information
Performing Location Reference Range
Marshfield​

Albumin:  3.70-4.90 g/dL

Alpha-1 globulin: 0.20-0.40 g/dL

Alpha-2 globulin: 0.50-0.90 g/dL

Beta-globulin: 0.60- 1.00 g/dL

Gamma-globulin: 0.60-1.40 g/dL

 

Refer to link provided for Protein, Total Reference Ranges: 

Protein, Total

Refer to link provided for Immunoglobulin Reference Ranges:

Immunoglobulin Reference Ranges

Interpretation
Increased serum immunoglobulin concentrations occur due to polyclonal or oligoclonal immunoglobulin proliferation in hepatic disease (hepatitis, liver cirrhosis), connective tissue diseases, acute and chronic infections, as well as in the cord blood of neonates with intra uterine and perinatal infections. Elevations of IgG, IgA, or IgM may occur in monoclonal gammopathies such as multiple myeloma (IgG, IgA), macroglobulinemia (IgM), primary systemic amyloidosis, monoclonal gammopathy of undetermined significance (MGUS), and related disorders.
Decreased levels are found in patients with primary or secondary immune deficiencies.​
For more information visit:
Performing Laboratory Information
 
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​

6-8 hours

If Immunotyping is required - an additional 24 hours should be expected​

Protein Electrophoresis – Capillary electrophoresis / Sebia Capillarys 2 protein system​

Immunoglobulins – Turbidimetric method / The Binding Site, Optilite analyzer

Immunotyping electrophoresis/Sebia Capillarys 2 Protein System​

Total serum protein: Biuret Blanked Endpoint Assay/Beckman AU 

For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
84165​ ​ ​ Protein electrophoresis​ ​
82784​ ​ 3​ Quantitative immunoglobulins (IgG, IgA, IgM)​ ​
86334​ ​ ​ Immunotyping (if required)​ ​

​84155
​​​Total serum protein​
​
For most current information refer to the Marshfield Laboratory online reference manual.