Evaluation of suspected heredity sherocytosis associated hemolytic anemia
Confirming or detecting mild spherocytosis
Specimens must arrive within 72 hours of draw.
Immediately refrigerate specimen after draw to 0 to 4 degrees C. Do not aliquot or let samples freeze. Ship specimens in original tubes. Specimen and control should be stored and transported together at refrigerate temperature.
Patient age and sex are required.
Spherocytosis of any cause will result in increased osmotic fragility. Infrequently, other congenital hemolytic disorders may also be associated with positive results, as in patients with congenital nonspherocytic hemolytic anemia due to RBC enzyme deficiencies.
Patients with an immunohemolytic anemia, or who have recently received a blood transfusion may also have increased RBC lysis.
The shipping control specimen is required to adequately interpret these cases, as temperature extremes can increase the fragility of the specimen and cause false-positive results.
Resulting Cautions: Osmotic fragility results will be reported if the shipping control is normal.
If the shipping control is abnormal and the osmotic fragility results on the patient are within normal range, the results will be reported; however, a comment will be added to the report indicating that the shipping control was not entirely satisfactory.
The test will be cancelled if the patient specimen and shipping control are both abnormal.
> or =12 months: 0.50 g/dL NaCl (unincubated): 3-53% hemolysis 0.60 g/dL NaCl (incubated): 14-74% hemolysis 0.65 g/dL NaCl (incubated): 4-40% hemolysis 0.75 g/dL NaCl (incubated): 1-11% hemolysis
Reference values have not been established for patients who are <12 months of age.