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25573 Arbovirus IgM Antibody, Diagnostic Panel (SS02201)

Arbovirus IgM Antibody, Diagnostic Panel (SS02201)
Test Code: ARBOMSO
Synonyms/Keywords
​​Eastern Equine Encephalitis, St. Louis Encephalitis, La Crosse Encephalitis, West Nile Virus, Jamestown Canyon Virus and Powassan Virus.​
Test Components
Qualitative IgM testing for Eastern Equine Encephalitis, St. Louis Encephalitis, La Crosse Encephalitis, West Nile Virus, Jamestown Canyon Virus and Powassan Virus.
Useful For

Serodiagnosis of a recent infection with any of these agents in individuals with symptoms of arbovirus encephalitis with no other laboratory diagnosis.

Testing will not be performed as a screening test or on patients without symptoms of arbovirus infection.

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​CSF ​Sterile Container/Tube ​ ​3.0 mL ​2.0 mL ​
​No ​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​3.0 mL ​1.0 mL ​
Collection Processing Instructions
​PROVIDE ONSET DATE, TRAVEL HISTORY AND SYMPTOMS.  Specimens should be collected in the acute phase of illness.  Store and transport to WSLH at 2-8 degrees Celsius (refrigerated with cool packs). 

If submitting CSF, a serum sample must also be sent for the same testing. Order two ARBOMSO tests, one for CSF and one for serum.  Testing will not be performed if only CSF is submitted. Must collect serum sample within 48 hours of CSF sample collection. Alternatively, serum may be submitted by itself. Indicate on sample and order if the sample is CSF or serum.  
Specimen Stability Information
Specimen Type Temperature Time
​CSF ​
 ​Refrigerated
 48 hours
​Frozen
​>48 hours
​Serum ​
 ​Refrigerated​
 ​48 hours
​Frozen
​>48 hours
Rejection Criteria
Room Temperature
Interference
Cross-reaction may occur between the arboviruses. Negative results on a single acute phase specimen do not rule out infection, as specimen may have been obtained prior to the development of an antibody response.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Wisconsin State Lab of Hygiene (WSLH) ​Once weekly ​2 to 8 days ​Qualitative IgM capture ELISA for JCV, POWV and LAC; Microsphere immunoassay (MIA) for WNV/SLE and EEE
Reference Lab
Wisconsin State Lab of Hygiene
Test Information
Laboratory regulations require the following minimum information to be provided on the requisition form for a specimen to be accepted for testing: Patient name or unique identifier; date and time of collection, patient date of birth and sex, specimen type/site of collection, test request(s), clinician name and UPIN, and address for reporting results. Please be certain that name/identifier on the form matches that on the specimen label.
Interpretation

​Negative, Presumptive Positive or Nonspecific for MIA assay.

Numeric value and interpretation as Negative, Equivocal, Presumptive Positive or Non-Specific for IgM capture ELISA. 

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86651 ​ ​1 ​La Crosse Encephalitis ​
​86652 ​ ​1 ​Eastern Equine Encephalitis ​
​86653 ​ ​1 ​St. Louis Encephalitis ​
​86788 ​ ​1 ​West Nile Virus IgM ​
​86790 ​ ​2 ​ ​
Synonyms/Keywords
​​Eastern Equine Encephalitis, St. Louis Encephalitis, La Crosse Encephalitis, West Nile Virus, Jamestown Canyon Virus and Powassan Virus.​
Test Components
Qualitative IgM testing for Eastern Equine Encephalitis, St. Louis Encephalitis, La Crosse Encephalitis, West Nile Virus, Jamestown Canyon Virus and Powassan Virus.
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​CSF ​Sterile Container/Tube ​ ​3.0 mL ​2.0 mL ​
​No ​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​3.0 mL ​1.0 mL ​
Collection Processing
​PROVIDE ONSET DATE, TRAVEL HISTORY AND SYMPTOMS.  Specimens should be collected in the acute phase of illness.  Store and transport to WSLH at 2-8 degrees Celsius (refrigerated with cool packs). 

If submitting CSF, a serum sample must also be sent for the same testing. Order two ARBOMSO tests, one for CSF and one for serum.  Testing will not be performed if only CSF is submitted. Must collect serum sample within 48 hours of CSF sample collection. Alternatively, serum may be submitted by itself. Indicate on sample and order if the sample is CSF or serum.  
Specimen Stability Information
Specimen Type Temperature Time
​CSF ​
 ​Refrigerated
 48 hours
​Frozen
​>48 hours
​Serum ​
 ​Refrigerated​
 ​48 hours
​Frozen
​>48 hours
Rejection Criteria
Room Temperature
Interference
Cross-reaction may occur between the arboviruses. Negative results on a single acute phase specimen do not rule out infection, as specimen may have been obtained prior to the development of an antibody response.
Useful For

Serodiagnosis of a recent infection with any of these agents in individuals with symptoms of arbovirus encephalitis with no other laboratory diagnosis.

Testing will not be performed as a screening test or on patients without symptoms of arbovirus infection.

Test Components
Qualitative IgM testing for Eastern Equine Encephalitis, St. Louis Encephalitis, La Crosse Encephalitis, West Nile Virus, Jamestown Canyon Virus and Powassan Virus.
Interpretation

​Negative, Presumptive Positive or Nonspecific for MIA assay.

Numeric value and interpretation as Negative, Equivocal, Presumptive Positive or Non-Specific for IgM capture ELISA. 

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Wisconsin State Lab of Hygiene (WSLH) ​Once weekly ​2 to 8 days ​Qualitative IgM capture ELISA for JCV, POWV and LAC; Microsphere immunoassay (MIA) for WNV/SLE and EEE
Reference Lab
Wisconsin State Lab of Hygiene
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86651 ​ ​1 ​La Crosse Encephalitis ​
​86652 ​ ​1 ​Eastern Equine Encephalitis ​
​86653 ​ ​1 ​St. Louis Encephalitis ​
​86788 ​ ​1 ​West Nile Virus IgM ​
​86790 ​ ​2 ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.