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25642 Leptospira, IgM, Serum (LEPDT)

Leptospira, IgM, Serum (LEPDT)
Test Code: LEPDTSO
Synonyms/Keywords
​L. biflexa, Leptospirosis
Useful For

​Aids in the diagnosis of leptospirosis

This test is intended to be used as an aid for the diagnosis of acute or recent leptospirosis due to infection with Leptospira species. 

This is a qualitative immunoblot test for detection of IgM-class antibodies to Leptospira species.  A negative result by this assay does not exclude the possibility of leptospirosis and all results must be correlated with clinical presentation and exposure history. 

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​Serum ​Serum Separator Tube (SST) ​Red Top Tube (RTT) ​0.3 mL ​0.1 mL ​
Collection Processing Instructions
​Serum should be collected according to standard practices.  Acute and convalescent specimens obtained to determine seroconversion should be collected 2 or more weeks apart.
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ ​Refrigerated (preferred) ​30 days
​Frozen ​30 days
Rejection Criteria
Hemolysis Gross reject
​Lipemia​Gross reject
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday, Wednesday , Friday ​1 to 5  days ​Enzyme-Linked Immunoassay Dot (Immunoblot)
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Reference Values
​Negative
Interpretation

​Positive:  IgM antibodies to Leptospira species detected suggesting recent infection.  Antibody presence alone cannot be used to definitively diagnose acute infection, as antibodies from a prior exposure or infection may remain detectable for a prolonged period of time.

Borderline:  Result should be interpreted with caution.  Additional testing of a second, convalescent specimen is recommended.  If the specimen remains borderline reactive, a second serological method should be considered if leptospirosis infection is still suspected.

Negative:  No IgM antibodies to Leptospira detected.  Since antibodies may not be present or may be present at undetectable levels during early disease, repeat testing of a convalescent sample collected in 2 to 3 weeks is recommended.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86720 ​ ​1 ​ ​
Synonyms/Keywords
​L. biflexa, Leptospirosis
Ordering Applications
Ordering Application Description

​Cerner
​Leptospira, IgM, S (LEPDT)​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​Serum ​Serum Separator Tube (SST) ​Red Top Tube (RTT) ​0.3 mL ​0.1 mL ​
Collection Processing
​Serum should be collected according to standard practices.  Acute and convalescent specimens obtained to determine seroconversion should be collected 2 or more weeks apart.
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ ​Refrigerated (preferred) ​30 days
​Frozen ​30 days
Rejection Criteria
Hemolysis Gross reject
​Lipemia​Gross reject
Useful For

​Aids in the diagnosis of leptospirosis

This test is intended to be used as an aid for the diagnosis of acute or recent leptospirosis due to infection with Leptospira species. 

This is a qualitative immunoblot test for detection of IgM-class antibodies to Leptospira species.  A negative result by this assay does not exclude the possibility of leptospirosis and all results must be correlated with clinical presentation and exposure history. 

Reference Range Information
Reference Values
​Negative
Interpretation

​Positive:  IgM antibodies to Leptospira species detected suggesting recent infection.  Antibody presence alone cannot be used to definitively diagnose acute infection, as antibodies from a prior exposure or infection may remain detectable for a prolonged period of time.

Borderline:  Result should be interpreted with caution.  Additional testing of a second, convalescent specimen is recommended.  If the specimen remains borderline reactive, a second serological method should be considered if leptospirosis infection is still suspected.

Negative:  No IgM antibodies to Leptospira detected.  Since antibodies may not be present or may be present at undetectable levels during early disease, repeat testing of a convalescent sample collected in 2 to 3 weeks is recommended.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday, Wednesday , Friday ​1 to 5  days ​Enzyme-Linked Immunoassay Dot (Immunoblot)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​86720 ​ ​1 ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.