Skip Ribbon Commands
Skip to main content
Sign In

26000 1p19q Deletion in Gliomas, FISH, Tissue (GLIOF)

1p19q Deletion in Gliomas, FISH, Tissue (GLIOF)
Test Code: FGLIOSO
Synonyms/Keywords
Oligodendroglioma
Useful For

Aids in diagnosing oligodendroglioma tumors and predicting the response of an oligodendroglioma to therapy

May be useful in tumors with a complex "hybrid" morphology requiring differentiation from pure astrocytomas to support the presence of oligodendroglial differentiation/lineage

Indicated when a diagnosis of oligodendroglioma, both low-grade World Health Organization (WHO, grade II) and anaplastic (WHO, grade III) is rendered

Strongly recommended when a diagnosis of mixed oligoastrocytomas is rendered

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​​Tissue ​Tissue Block
​No ​Slides​Six consecutive, unstained and 1 hematoxylin and eosin-stained slide
Collection Processing Instructions

Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

OR

Six consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

A reason for referral and pathology report are required in order for testing to be performed. Send information with specimen. Acceptable pathology reports include working drafts, preliminary pathology or surgical pathology reports.

Specimen Stability Information
Specimen Type Temperature
​Tissue ​ ​Ambient (preferred)
​Refrigerated
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Sunday ​8 days Fluorescence In Situ Hybridization (​FISH) using DNA probes
Reference Lab
Reference Range Information
An interpretive report will be provided. 
Interpretation

​The presence of 1p deletion and combined 1p and 19q deletion supports a diagnosis of oligodendroglioma may indicate that the patient may respond to chemotherapy and radiation therapy.

The presence of gain of chromosome 19 supports a diagnosis of high-grade astrocytoma (glioblastoma multiforme).

A negative result does not exclude a diagnosis of oligodendroglioma or high-grade astrocytoma.

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​88271 2 ​DNA probe, each
​88291 ​1 ​Interpretation and Report
​88271 1 ​Probe, +1 ​if needed
​88271 2 ​​Probe, +2 ​if needed
​88271 ​3 ​Probe, +3 ​if needed
​88271 ​2 ​Probe set, count ​if needed
​88274 ​1 ​​Interphases, 25-99 ​if needed
​88275 ​1 ​​Interphases, 100-300 ​if needed
88274​ ​1 ​​Interphases, <25 ​​if needed
Synonyms/Keywords
Oligodendroglioma
Ordering Applications
Ordering Application Description
​All ​1p19q Deletion in Gliomas, FISH, Tissue (GLIOF)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​​Tissue ​Tissue Block
​No ​Slides​Six consecutive, unstained and 1 hematoxylin and eosin-stained slide
Collection Processing

Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

OR

Six consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

A reason for referral and pathology report are required in order for testing to be performed. Send information with specimen. Acceptable pathology reports include working drafts, preliminary pathology or surgical pathology reports.

Specimen Stability Information
Specimen Type Temperature
​Tissue ​ ​Ambient (preferred)
​Refrigerated
Useful For

Aids in diagnosing oligodendroglioma tumors and predicting the response of an oligodendroglioma to therapy

May be useful in tumors with a complex "hybrid" morphology requiring differentiation from pure astrocytomas to support the presence of oligodendroglial differentiation/lineage

Indicated when a diagnosis of oligodendroglioma, both low-grade World Health Organization (WHO, grade II) and anaplastic (WHO, grade III) is rendered

Strongly recommended when a diagnosis of mixed oligoastrocytomas is rendered

Reference Range Information
An interpretive report will be provided. 
Interpretation

​The presence of 1p deletion and combined 1p and 19q deletion supports a diagnosis of oligodendroglioma may indicate that the patient may respond to chemotherapy and radiation therapy.

The presence of gain of chromosome 19 supports a diagnosis of high-grade astrocytoma (glioblastoma multiforme).

A negative result does not exclude a diagnosis of oligodendroglioma or high-grade astrocytoma.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Sunday ​8 days Fluorescence In Situ Hybridization (​FISH) using DNA probes
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​88271 2 ​DNA probe, each
​88291 ​1 ​Interpretation and Report
​88271 1 ​Probe, +1 ​if needed
​88271 2 ​​Probe, +2 ​if needed
​88271 ​3 ​Probe, +3 ​if needed
​88271 ​2 ​Probe set, count ​if needed
​88274 ​1 ​​Interphases, 25-99 ​if needed
​88275 ​1 ​​Interphases, 100-300 ​if needed
88274​ ​1 ​​Interphases, <25 ​​if needed
For most current information refer to the Marshfield Laboratory online reference manual.