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26034 Hepatitis B Surface Antigen, DaVita Dialysis

Hepatitis B Surface Antigen, DaVita Dialysis
Test Code: HBSAGDV
Synonyms/Keywords
DaVita, Dialysis, Hepatitis, HBSAG
Useful For

​The HBSAGDV is an alternate HBsAg test code to be used specifically for DaVita Dialysis patient testing, and is available only to Marshfield Medical Center and the Diagnostic and Treatment Center in Weston. 

For routine Hepatitis B Surface Antigen (HBsAg) requests, see HAA test code.  The assay is used to aid in the diagnosis of hepatitis B and determiing the phase of a hepatitis B infection.

Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​Serum ​Serum Separator  Tube (SST) ​Red Top Tube (RTT) ​1.0 mL ​0.5 mL ​0.5 mL
Collection Processing Instructions
​Separate serum from the blood within 24 hours of collection, and transport in an aliquot tube.  Store serum specimens at 2-8°C if not to be tested within 24 hours of collection.  Specimen must be free of particulate matter including fibrin.  For longer storage, freeze specimens at -20°C or colder.  Frozen specimens should be completely thawed, throroughly mixed and centrifuged before analysis.  Up to 4 freeze thaw cycles is acceptable.
 
For U-Have testing, specimens stored in primary tubes up to 14 days at 2-8°C are acceptable.  Primary tubes include serum stored on the clot, and samples processed and stored in gel barrier blood collection tubes.
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay.  It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ ​ ​ ​Ambient ​24 hours
​Refrigerate ​14 days
​Frozen at -20°C ​6 months
​Frozen at -70°C ​>6 months
Rejection Criteria
Grossly hemolyzed
​Grossly icteric
​Specimens containing precipitate
​Cadaver specimens
​Heat-inactivated specimens
​Body fluids other than serum
Interference
​Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with immunoassay. 
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday
​Initial result:  < 2 hours
Confirmation of Reactives:  3 days
 Sandwich immunoassay using Direct Chemiluminometric Technology/Siemens Centaur
Test Information

​Hepatitis B is a viral illness that is transmitted through blood and other body fluids.  Both acute and chronic stages occur, with the frequency of progression to chronicity declining with age at time of infection.  Chronic hepatitis B often leads to cirrhosis and hepatocellular carcinoma.  Hepatitis B is a vaccine-preventable disease. 

Limitations:

•  The results determined by different assays from different manufacturers can vary due to different assay specificities and cannot be used interchangeably.
•  It is recognized that the current methods for the detection of hepatitis B surface antigen may not detect all potentially infected individuals.  A nonreactive test result does not exclude the possibility of exposure to or infection with hepatitis B virus.  A nonreactive test result in individuals with prior exposure to hepatitis B may be due to antigen levels below the detection limit of this assay or lack of antigen reactivity to the antibodies in this assay.
•  Assay performance characteristics have not been established in populations of immunocompromised or immunosuppressed patients.
Reference Range Information
Performing Location Reference Range
​Marshfield ​Non-Reactive
Interpretation
​The presence of hepatitis B surface antigen indicates ongoing acute or chronic hepatitis B infection. 
A result of a single hepatitis B lab study should be interpreted with caution.
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87340 ​ ​ ​ ​
Synonyms/Keywords
DaVita, Dialysis, Hepatitis, HBSAG
Ordering Applications
Ordering Application Description
​CENTRICITY ​Hepatitis B Surface Ag, DaVita
​COM ​Hepatitis B Surface Ag, DaVita
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
​No ​Serum ​Serum Separator  Tube (SST) ​Red Top Tube (RTT) ​1.0 mL ​0.5 mL ​0.5 mL
Collection Processing
​Separate serum from the blood within 24 hours of collection, and transport in an aliquot tube.  Store serum specimens at 2-8°C if not to be tested within 24 hours of collection.  Specimen must be free of particulate matter including fibrin.  For longer storage, freeze specimens at -20°C or colder.  Frozen specimens should be completely thawed, throroughly mixed and centrifuged before analysis.  Up to 4 freeze thaw cycles is acceptable.
 
For U-Have testing, specimens stored in primary tubes up to 14 days at 2-8°C are acceptable.  Primary tubes include serum stored on the clot, and samples processed and stored in gel barrier blood collection tubes.
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay.  It is recommended that patients refrain from consuming any multivitamin or supplement containing biotin for at least 72 hours prior to collection of a blood sample.
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ ​ ​ ​Ambient ​24 hours
​Refrigerate ​14 days
​Frozen at -20°C ​6 months
​Frozen at -70°C ​>6 months
Rejection Criteria
Grossly hemolyzed
​Grossly icteric
​Specimens containing precipitate
​Cadaver specimens
​Heat-inactivated specimens
​Body fluids other than serum
Interference
​Heterophilic antibodies in human serum can react with the immunoglobulins included in the assay components causing interference with immunoassay. 
 
High doses of exogenous biotin (also termed Vitamin B7, Vitamin H or Coenzyme R) may interfere with this assay.
Useful For

​The HBSAGDV is an alternate HBsAg test code to be used specifically for DaVita Dialysis patient testing, and is available only to Marshfield Medical Center and the Diagnostic and Treatment Center in Weston. 

For routine Hepatitis B Surface Antigen (HBsAg) requests, see HAA test code.  The assay is used to aid in the diagnosis of hepatitis B and determiing the phase of a hepatitis B infection.

Reference Range Information
Performing Location Reference Range
​Marshfield ​Non-Reactive
Interpretation
​The presence of hepatitis B surface antigen indicates ongoing acute or chronic hepatitis B infection. 
A result of a single hepatitis B lab study should be interpreted with caution.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday
​Initial result:  < 2 hours
Confirmation of Reactives:  3 days
 Sandwich immunoassay using Direct Chemiluminometric Technology/Siemens Centaur
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
​87340 ​ ​ ​ ​
For most current information refer to the Marshfield Laboratory online reference manual.