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26311 Heparin Induced Thrombocytopenia Antibodies

Heparin Induced Thrombocytopenia Antibodies
Test Code: HITAB
Synonyms/Keywords

​Heparin Induced Antibody, HIT, PF4

Test Components

​HIT antibody

Note: Testing will not be performed if patient has platelet counts >= 150,000.  Testing will be paused, the sample will be held for 5 days, after which testing will be cancelled and credited.  Please contact the lab for approval for testing of patients without thrombocytopenia.

Useful For

​Diagnosing heparin-induced thrombocytopenia.

Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​No​Citrated Plasma​Citrated Blue Top Tube (BTT)​​3 mL​1.0 mL​
Collection Processing Instructions

Collect two citrated Blue Top Tubes (BTT) to make two 1.0 mL platelet poor plasma aliquots

If other coagulation testing is ordered, separate aliquots are needed for each individual test

Citrate anticoagulant must be adjusted for HCT >55%

Tube must be at least 90% full

Invert completely 3-4 times (without shaking) to mix

*See Instructions for: Preparation of Platelet Poor Plasma 

Specimen Stability Information
Specimen TypeTemperatureTime
​Plasma ​​Ambient​4 hours
​Frozen (-70 degrees C)​6 months
Rejection Criteria
Clotted or Hemolyzed
​Contamination with IV or Hickman Line Fluids
​Specimens that thaw during storage or transport
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Marshfield

​Monday through Friday - twice per day

Weekends/Holidays - as needed

Less than 2 hours

​ACL TOP 550
Reference Range Information
Performing LocationReference Range
​Marshfield​

Negative: This result has approximately 90-95% negative predictive value for excluding heparin-induced thrombocytopenia (HIT)

Positive:  Heparin dependent antibodies are present. This result has a reasonably good correlation with the serotonin release assay (SRA). Serotonin Release Assay (SRA) has been ordered and will be performed by Versiti. Results should be available within 2-3 days. If the clinical situation warrants, initiating treatment for heparin-induced thrombocytopenia (HIT) is reasonable.

Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​86022​​1​Heparin antibodies​
​86022​​1​Serotonin, send-out to Versiti​if needed
Synonyms/Keywords

​Heparin Induced Antibody, HIT, PF4

Test Components

​HIT antibody

Note: Testing will not be performed if patient has platelet counts >= 150,000.  Testing will be paused, the sample will be held for 5 days, after which testing will be cancelled and credited.  Please contact the lab for approval for testing of patients without thrombocytopenia.

Ordering Applications
Ordering ApplicationDescription
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​No​Citrated Plasma​Citrated Blue Top Tube (BTT)​​3 mL​1.0 mL​
Collection Processing

Collect two citrated Blue Top Tubes (BTT) to make two 1.0 mL platelet poor plasma aliquots

If other coagulation testing is ordered, separate aliquots are needed for each individual test

Citrate anticoagulant must be adjusted for HCT >55%

Tube must be at least 90% full

Invert completely 3-4 times (without shaking) to mix

*See Instructions for: Preparation of Platelet Poor Plasma 

Specimen Stability Information
Specimen TypeTemperatureTime
​Plasma ​​Ambient​4 hours
​Frozen (-70 degrees C)​6 months
Rejection Criteria
Clotted or Hemolyzed
​Contamination with IV or Hickman Line Fluids
​Specimens that thaw during storage or transport
Useful For

​Diagnosing heparin-induced thrombocytopenia.

Test Components

​HIT antibody

Note: Testing will not be performed if patient has platelet counts >= 150,000.  Testing will be paused, the sample will be held for 5 days, after which testing will be cancelled and credited.  Please contact the lab for approval for testing of patients without thrombocytopenia.

Reference Range Information
Performing LocationReference Range
​Marshfield​

Negative: This result has approximately 90-95% negative predictive value for excluding heparin-induced thrombocytopenia (HIT)

Positive:  Heparin dependent antibodies are present. This result has a reasonably good correlation with the serotonin release assay (SRA). Serotonin Release Assay (SRA) has been ordered and will be performed by Versiti. Results should be available within 2-3 days. If the clinical situation warrants, initiating treatment for heparin-induced thrombocytopenia (HIT) is reasonable.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Marshfield

​Monday through Friday - twice per day

Weekends/Holidays - as needed

Less than 2 hours

​ACL TOP 550
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​86022​​1​Heparin antibodies​
​86022​​1​Serotonin, send-out to Versiti​if needed
For most current information refer to the Marshfield Laboratory online reference manual.