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26401 Low-Grade Fibromyxoid Sarcoma (LGFMS), 16p11.2 (FUS or TLS) Rearrangement, FISH, Tissue (FUSF)

Low-Grade Fibromyxoid Sarcoma (LGFMS), 16p11.2 (FUS or TLS) Rearrangement, FISH, Tissue (FUSF)
Test Code: FUSFSO
Test Components

TESTING ALGORITHM: 

This test does not include a pathology consult. If a pathology consultation is requested, PATHC / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge.

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for application of all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred. 

Useful For

​Supporting the diagnosis of low-grade fibromyxoid sarcoma when used in conjunction with an anatomic pathology consultation.

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
​Tissue​Tissue Block - FFPE​Slides​See collection instructions​Two consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.
Collection Processing Instructions

​Tissue Block: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

Slides: Four consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

NECESSARY INFORMATION:

1. A pathology report is required in order for testing to be performed. Acceptable pathology reports include working drafts, preliminary pathology or surgical pathology reports.

2. A reason for testing must be provided. If this information is not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.

Specimen Stability Information
Specimen TypeTemperature
​Tissue ​​Ambient (preferred)
​Refrigerated
Rejection Criteria
All specimens will be evaluated by Mayo Clinic Laboratories for test suitability. 
Interference

​This test is not approved by the US Food and Drug Administration  and it is best used as an adjunct to existing clinical and pathologic information.

Fixatives other than formalin (eg, Prefer, Bouin) may not be successful for FISH assays, however nonformalin-fixed samples will not be rejected.

Paraffin-embedded tissues that have been decalcified are generally unsuccessful for FISH analysis. The pathologist reviewing the hematoxylin and eosin-stained slide may find it necessary to cancel testing.

Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​7 to 10 days​Fluorescence In Situ Hybridization (FISH)
Reference Lab
Mayo Clinic Laboratories
Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​An interpretative report will be provided. 
Interpretation

​A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal cutoff for the FUS probe set.

A positive result is consistent with the diagnosis of low-grade fibromyxoid sarcoma (LGFMS).

A negative result suggests that a FUS gene rearrangement is not present, but does not exclude the diagnosis of LGFMS.

Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​88271​​2​​
​88291​​1​​
​88271​​2​each additional probe set​if appropriate
​88271​​1​coverage for sets containing 3 probes​if appropriate
​88271​​2​coverage for sets containing 4 probes​if appropriate
​88271​​3​coverage for sets containing 5 probes​if appropriate
​88274​​1​< 25 cells, each probe set​if appropriate
​88274​​1​25 to 99 cells, each probe set​if appropriate
Test Components

TESTING ALGORITHM: 

This test does not include a pathology consult. If a pathology consultation is requested, PATHC / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge.

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for application of all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred. 

Ordering Applications
Ordering ApplicationDescription
​COM​Low-Grade Fibromyxoid Sarcoma Rearran FISH (FUSF)
​Cernger​Low-Grade Fibromyxoid Sarcoma (LGFMS), 16p11.2 (FUS or TLS) Rearrangement, FISH Tissue (FUSF)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
​Tissue​Tissue Block - FFPE​Slides​See collection instructions​Two consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.
Collection Processing

​Tissue Block: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

Slides: Four consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

NECESSARY INFORMATION:

1. A pathology report is required in order for testing to be performed. Acceptable pathology reports include working drafts, preliminary pathology or surgical pathology reports.

2. A reason for testing must be provided. If this information is not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.

Specimen Stability Information
Specimen TypeTemperature
​Tissue ​​Ambient (preferred)
​Refrigerated
Rejection Criteria
All specimens will be evaluated by Mayo Clinic Laboratories for test suitability. 
Interference

​This test is not approved by the US Food and Drug Administration  and it is best used as an adjunct to existing clinical and pathologic information.

Fixatives other than formalin (eg, Prefer, Bouin) may not be successful for FISH assays, however nonformalin-fixed samples will not be rejected.

Paraffin-embedded tissues that have been decalcified are generally unsuccessful for FISH analysis. The pathologist reviewing the hematoxylin and eosin-stained slide may find it necessary to cancel testing.

Useful For

​Supporting the diagnosis of low-grade fibromyxoid sarcoma when used in conjunction with an anatomic pathology consultation.

Test Components

TESTING ALGORITHM: 

This test does not include a pathology consult. If a pathology consultation is requested, PATHC / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge.

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for application of all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred. 

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​An interpretative report will be provided. 
Interpretation

​A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal cutoff for the FUS probe set.

A positive result is consistent with the diagnosis of low-grade fibromyxoid sarcoma (LGFMS).

A negative result suggests that a FUS gene rearrangement is not present, but does not exclude the diagnosis of LGFMS.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​7 to 10 days​Fluorescence In Situ Hybridization (FISH)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​88271​​2​​
​88291​​1​​
​88271​​2​each additional probe set​if appropriate
​88271​​1​coverage for sets containing 3 probes​if appropriate
​88271​​2​coverage for sets containing 4 probes​if appropriate
​88271​​3​coverage for sets containing 5 probes​if appropriate
​88274​​1​< 25 cells, each probe set​if appropriate
​88274​​1​25 to 99 cells, each probe set​if appropriate
For most current information refer to the Marshfield Laboratory online reference manual.