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26429 Antimicrobial Susceptibility, Acid-Fast Bacilli, Rapidly Growing, Varies (MMLRG)

Antimicrobial Susceptibility, Acid-Fast Bacilli, Rapidly Growing, Varies (MMLRG)
Test Code: MISC
Synonyms/Keywords

​Acid-Fast Bacilli (AFB), AFB (Acid-Fast Bacilli), Antibiotic Susceptibility, Bacillus, Acid-Fast, MIC (Minimum Inhibitory Concentration), Minimum Inhibitory Concentration (MIC), Mycobacteria Antimicrobial Susceptibility (MIC) (Minimum Inhibitory Concentration), Mycobacterium abscessus Susceptibility Testing, Mycobacterium chelonae Susceptibility Testing, Mycobacterium fortuitum Susceptibility Testing, Mycobacterium mucogenicum Susceptibility Testing, Rapid Growing Mycobacteria to Antimicrobial Agents, Susceptibility Testing, Clofazimine

Test Components

Antimicrobials tested in this assay are amikacin, cefoxitin, ciprofloxacin, clarithromycin, clofazimine, doxycycline, imipenem, linezolid, moxifloxacin, tigecycline, tobramycin, and trimethoprim/sulfamethoxazole.

Useful For

​Determination of susceptibility of rapidly growing mycobacteria to the antimicrobial agents on the test panel.

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Organism​Middlebrook 7H10 agar slant or other appropriate media​Pure Isolate
Collection Processing Instructions

​Organism must be in pure culture, actively growing on Middlebrook 7H10 agar slant or other appropriate media. 

​CTB / Mycobacteria and Nocardia Culture, Varies or CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies must also be ordered and will be charged separately unless identification of organism is provided by the client.

Specimen source is required.

Identification to the species level is required for Mycobacterium species in order for the correct antimicrobial susceptibility drug panel to be selected. Identification to the genus level is sufficient for Nocardia species and other aerobic actinomycetes (eg, Gordonia species, Rhodococcus species).

Shipping Instructions

1. See Infectious Specimen Shipping Guidelines in Special Instructions.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.

Specimen Stability Information
Specimen TypeTemperatureTime
Varies​​Ambient (preferred)
​Refrigerated
Rejection Criteria

Agar plate

Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
Mayo Clinic Laboratories​​Monday through Sunday​12-28 days​Minimum Inhibitory Concentration (MIC) by Microtiter Broth Dilution Method
Reference Lab
Test Information

​There are more than 100 species of rapidly growing mycobacteria and many are significant human pathogens (eg, Mycobacterium abscessus, Mycobacterium chelonae, Mycobacterium fortuitum). Rapidly growing mycobacteria cause a variety of infections including pulmonary infections, skin and soft tissue infections, and disseminated disease. Antimicrobial susceptibility testing of clinically significant rapidly growing mycobacteria is important to help guide patient care.

Antimicrobials tested in this assay are amikacin, cefoxitin, ciprofloxacin, clarithromycin, clofazimine, doxycycline, imipenem, linezolid, moxifloxacin, tigecycline, tobramycin, and trimethoprim/sulfamethoxazole.

Reference Range Information
 
Antimicrobial
Susceptible (mcg/mL)Intermediate (mcg/mL)Resistant (mcg/mL)
Amikacin< or =1632> or =64
Cefoxitin< or =1632-64> or =128
Ciprofloxacin< or =12> or =4
Clarithromycin< or =24> or =8
ClofazimineNo interpretations available
Doxycycline < or =12-4> or =8
Imipenem< or =48-16> or =32
Linezolid< or =816> or =32
Moxifloxacin< or =12> or =4
TigecyclineNo interpretations available
Tobramycin< or =24> or =8
Trimethoprim/Sulfamethoxazole< or =2/38-> or =4/76
Interpretation

​Results are reported as the minimum inhibitory concentration in micrograms/mL. Interpretive criteria (susceptible, intermediate, or resistant) are reported according the Clinical and Laboratory Standards Institute (CLSI) guidelines.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87186​1
Synonyms/Keywords

​Acid-Fast Bacilli (AFB), AFB (Acid-Fast Bacilli), Antibiotic Susceptibility, Bacillus, Acid-Fast, MIC (Minimum Inhibitory Concentration), Minimum Inhibitory Concentration (MIC), Mycobacteria Antimicrobial Susceptibility (MIC) (Minimum Inhibitory Concentration), Mycobacterium abscessus Susceptibility Testing, Mycobacterium chelonae Susceptibility Testing, Mycobacterium fortuitum Susceptibility Testing, Mycobacterium mucogenicum Susceptibility Testing, Rapid Growing Mycobacteria to Antimicrobial Agents, Susceptibility Testing, Clofazimine

Test Components

Antimicrobials tested in this assay are amikacin, cefoxitin, ciprofloxacin, clarithromycin, clofazimine, doxycycline, imipenem, linezolid, moxifloxacin, tigecycline, tobramycin, and trimethoprim/sulfamethoxazole.

Ordering Applications
Ordering ApplicationDescription
​COM

​Antimicr SuscAF Bacilli, Rapidly Growing (MMLRG)

​Cerner​Antimicrobial Susceptibility, Acid-Fast Bacilli, Rapidly Growing, Varies (MMLRG)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Organism​Middlebrook 7H10 agar slant or other appropriate media​Pure Isolate
Collection Processing

​Organism must be in pure culture, actively growing on Middlebrook 7H10 agar slant or other appropriate media. 

​CTB / Mycobacteria and Nocardia Culture, Varies or CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies must also be ordered and will be charged separately unless identification of organism is provided by the client.

Specimen source is required.

Identification to the species level is required for Mycobacterium species in order for the correct antimicrobial susceptibility drug panel to be selected. Identification to the genus level is sufficient for Nocardia species and other aerobic actinomycetes (eg, Gordonia species, Rhodococcus species).

Shipping Instructions

1. See Infectious Specimen Shipping Guidelines in Special Instructions.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.

Specimen Stability Information
Specimen TypeTemperatureTime
Varies​​Ambient (preferred)
​Refrigerated
Rejection Criteria

Agar plate

Useful For

​Determination of susceptibility of rapidly growing mycobacteria to the antimicrobial agents on the test panel.

Test Components

Antimicrobials tested in this assay are amikacin, cefoxitin, ciprofloxacin, clarithromycin, clofazimine, doxycycline, imipenem, linezolid, moxifloxacin, tigecycline, tobramycin, and trimethoprim/sulfamethoxazole.

Reference Range Information
 
Antimicrobial
Susceptible (mcg/mL)Intermediate (mcg/mL)Resistant (mcg/mL)
Amikacin< or =1632> or =64
Cefoxitin< or =1632-64> or =128
Ciprofloxacin< or =12> or =4
Clarithromycin< or =24> or =8
ClofazimineNo interpretations available
Doxycycline < or =12-4> or =8
Imipenem< or =48-16> or =32
Linezolid< or =816> or =32
Moxifloxacin< or =12> or =4
TigecyclineNo interpretations available
Tobramycin< or =24> or =8
Trimethoprim/Sulfamethoxazole< or =2/38-> or =4/76
Interpretation

​Results are reported as the minimum inhibitory concentration in micrograms/mL. Interpretive criteria (susceptible, intermediate, or resistant) are reported according the Clinical and Laboratory Standards Institute (CLSI) guidelines.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
Mayo Clinic Laboratories​​Monday through Sunday​12-28 days​Minimum Inhibitory Concentration (MIC) by Microtiter Broth Dilution Method
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87186​1
For most current information refer to the Marshfield Laboratory online reference manual.