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26439 MYD88, L265P, Somatic Gene Mutation, DNA Allele-Specific PCR, Varies (MYD88)

MYD88, L265P, Somatic Gene Mutation, DNA Allele-Specific PCR, Varies (MYD88)
Test Code: MYD88SO
Synonyms/Keywords

​MYD88, L265, Somatic Gene Mutation, DNA Allele-Specific PCR, Varies (MYD88), MYD88, L265P, B-cell lymphoma, LPL/WM, Lymphoplasmacytic lymphoma, Waldenstrom's macroglobulinemia

Useful For

​Establishing the diagnosis of lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia

Helping to distinguish lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia (low-grade B-cell lymphoma) from other subtypes

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​Bone Marrow​Lavender Top EDTA

​Yellow Top (ACD solution B)

or

Green Top Tube (Heparin)

​2 mL​​
​Paraffin-embedded tissue​Paraffin block​​​​
​Paraffin-embedded bone marrow aspirate clot​Paraffin block​​​​
​Peripheral blood​Lavender Top EDTA

​Yellow Top (ACD solution b)

or

Green Top Tube (Heparin)

​3 mL​​
​Frozen tissue​Plastic container​​100 mg​​
​Unstained slides​Unstained tissue slides​​10 slides​​
​Extracted DNA​1.5 to 2 mL tube​​Entire specimen​​
​Methanol-acetic acid (MAA) fixed pellets​Plastic container​​​​
Collection Processing Instructions

Submit only 1 of the above specimens.

​Peripheral blood or bone marrow specimens must arrive within 10 days (240 hours) of collection.

The following information is required:

-Pertinent clinical history

-Clinical or morphologic suspicion

-Date of collection

-Specimen source

 

Collection Instructions for Bone Marrow:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube. Do not aliquot.

3. Label specimen as bone marrow.

Specimen Stability: Ambient (preferred)/Refrigerated

 

Paraffin-embedded tissue and Paraffin-embedded bone marrow aspirate clot stability: Ambient

 

Collection Instructions for Peripheral blood:

1. Invert several times to mix blood.

2. Send specimen in original tube. Do not aliquot.

3. Label specimen as blood.

Specimen Stability: Ambient (preferred)/Refrigerated

 

Collection Instructions for Frozen tissue:

1. Freeze tissue within 1 hour of collection.

Specimen Stability: Frozen

 

Unstained slides stability: Ambient

 

Collection Instructions for Extracted DNA:

1. Label specimen as extracted DNA and source of specimen.

2. Indicate volume and concentration of the DNA on the label.

Specimen Stability: Frozen (preferred)/Refrigerated

 

Methanol-acetic acid (MAA) fixed pellets stability: Ambient (preferred)/Refrigerated

Specimen Stability Information
Specimen TypeTemperatureTime
​Varies​Varies (preferred)​10 Days
Rejection Criteria
Gross HemolysisReject
​Bone marrow core biopsies​Reject
​Paraffin shavings​Reject
​Moderately to severely clotted​Reject
Interference

​This test is a targeted assay and will not detect any alteration at the MYD88 codon 265 that does not result in the L>P (leucine to proline) amino acid change. It will also not detect additional MYD88 alterations, including insertion or deletion events. The analytical sensitivity of the assay (1% MYD88 L265P in a wild-type background) can be affected by a variety of factors, including biologic availability (ie, tumor burden), fixation of paraffin-embedded specimens, or nonspecific polymerase chain reaction interferences. Rare cases of lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia (LPL/WM) have been reported lacking the MYD88 L265P abnormality, so a negative result would not completely exclude this diagnosis but would make the possibility of LPL/WM more unlikely.

Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​3-5 Days​Allele-Specific Polymerase Chain Reaction (PCR)
Reference Lab
Mayo Clinic Laboratories
Test Information

​The single point alteration in MYD88, L265P, is present in 67% to 100% of patients with lymphoplasmacytic lymphoma, and these patients typically have clinical manifestations of Waldenstrom macroglobulinemia (often designated LPL/WM).

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Variant present or absent based on expected alteration polymerase chain reaction product size. Concurrent amplification of wild type MYD88 fragment determined for sample amplification integrity. MYD88 gene (NCBI accession NM_002468.4).
Interpretation

​Variant present or not detected; an interpretive report will be issued.

Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
81305​​​1​​
Synonyms/Keywords

​MYD88, L265, Somatic Gene Mutation, DNA Allele-Specific PCR, Varies (MYD88), MYD88, L265P, B-cell lymphoma, LPL/WM, Lymphoplasmacytic lymphoma, Waldenstrom's macroglobulinemia

Ordering Applications
Ordering ApplicationDescription
​COM​MYD88, L265P, Somatic Gene Mut PCR (MYD88)
​Cerner​MYD88, L265P, Somatic Gene Mutation, DNA Allele-Specific PCR, Varies (MYD88)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​Bone Marrow​Lavender Top EDTA

​Yellow Top (ACD solution B)

or

Green Top Tube (Heparin)

​2 mL​​
​Paraffin-embedded tissue​Paraffin block​​​​
​Paraffin-embedded bone marrow aspirate clot​Paraffin block​​​​
​Peripheral blood​Lavender Top EDTA

​Yellow Top (ACD solution b)

or

Green Top Tube (Heparin)

​3 mL​​
​Frozen tissue​Plastic container​​100 mg​​
​Unstained slides​Unstained tissue slides​​10 slides​​
​Extracted DNA​1.5 to 2 mL tube​​Entire specimen​​
​Methanol-acetic acid (MAA) fixed pellets​Plastic container​​​​
Collection Processing

Submit only 1 of the above specimens.

​Peripheral blood or bone marrow specimens must arrive within 10 days (240 hours) of collection.

The following information is required:

-Pertinent clinical history

-Clinical or morphologic suspicion

-Date of collection

-Specimen source

 

Collection Instructions for Bone Marrow:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube. Do not aliquot.

3. Label specimen as bone marrow.

Specimen Stability: Ambient (preferred)/Refrigerated

 

Paraffin-embedded tissue and Paraffin-embedded bone marrow aspirate clot stability: Ambient

 

Collection Instructions for Peripheral blood:

1. Invert several times to mix blood.

2. Send specimen in original tube. Do not aliquot.

3. Label specimen as blood.

Specimen Stability: Ambient (preferred)/Refrigerated

 

Collection Instructions for Frozen tissue:

1. Freeze tissue within 1 hour of collection.

Specimen Stability: Frozen

 

Unstained slides stability: Ambient

 

Collection Instructions for Extracted DNA:

1. Label specimen as extracted DNA and source of specimen.

2. Indicate volume and concentration of the DNA on the label.

Specimen Stability: Frozen (preferred)/Refrigerated

 

Methanol-acetic acid (MAA) fixed pellets stability: Ambient (preferred)/Refrigerated

Specimen Stability Information
Specimen TypeTemperatureTime
​Varies​Varies (preferred)​10 Days
Rejection Criteria
Gross HemolysisReject
​Bone marrow core biopsies​Reject
​Paraffin shavings​Reject
​Moderately to severely clotted​Reject
Interference

​This test is a targeted assay and will not detect any alteration at the MYD88 codon 265 that does not result in the L>P (leucine to proline) amino acid change. It will also not detect additional MYD88 alterations, including insertion or deletion events. The analytical sensitivity of the assay (1% MYD88 L265P in a wild-type background) can be affected by a variety of factors, including biologic availability (ie, tumor burden), fixation of paraffin-embedded specimens, or nonspecific polymerase chain reaction interferences. Rare cases of lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia (LPL/WM) have been reported lacking the MYD88 L265P abnormality, so a negative result would not completely exclude this diagnosis but would make the possibility of LPL/WM more unlikely.

Useful For

​Establishing the diagnosis of lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia

Helping to distinguish lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia (low-grade B-cell lymphoma) from other subtypes

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Variant present or absent based on expected alteration polymerase chain reaction product size. Concurrent amplification of wild type MYD88 fragment determined for sample amplification integrity. MYD88 gene (NCBI accession NM_002468.4).
Interpretation

​Variant present or not detected; an interpretive report will be issued.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​3-5 Days​Allele-Specific Polymerase Chain Reaction (PCR)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
81305​​​1​​
For most current information refer to the Marshfield Laboratory online reference manual.