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26446 Bile Alcohols, Urine

Bile Alcohols, Urine
Test Code: UBILALSO
Synonyms/Keywords

​URINARY BILE ALCOHOL 5b-CHOLESTANE-3a,7a,12a,23S,25-PENTOL, TOTAL

Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​​Random Urine​Urine Tube​​10.0 mL​2.0 mL​
Collection Processing Instructions

​Use Serol Analysis Lab requisition

No preservative

Specimen Stability Information
Specimen TypeTemperature​Time
​Urine ​ ​​Frozen (preferred)​Long Term
​Ambient​5 Days
​Refrigerated​11 Days
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Oregon Health & Science University​Batched Testing​2-4 Weeks​Addition of 23S-pentol-d6 method internal standard followed by incubation of urine with β-glucuronidase enzyme. Isotope-dilution quantification performed using positive-mode LC-ESI-MS/MS multiple reaction monitoring of 23S-pentol (m/z 453.3>361.4) and internal standard (m/z 459.3>343.5) with a reversed-phase gradient utilizing a 4.6x50mm Biphenyl (2.6 µm) column. Linear up to 5,000 ng/mL without dilution. No significant interference from common over the counter and prescription medications. Carry over response in blanks is < 1% of the LLOQ for the analyte and < 0.5% for the internal standard.
Reference Lab
Oregon Health & Science University - Physiology & Pharmacology Department (L334)
Reference Range Information

​

​Performing Location​Reference Range
​Oregon Health & Science University​

Normal concentration in unaffected individuals, n=20 < 200 ng/mL

Concentration in CTX-affected untreated individuals, n=8 67,322 ± 39,087 ng/mL [20,022-118,411] mean ± SD [range]

 

Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​82542​​1​​
Synonyms/Keywords

​URINARY BILE ALCOHOL 5b-CHOLESTANE-3a,7a,12a,23S,25-PENTOL, TOTAL

Ordering Applications
Ordering ApplicationDescription
​Cerner​Bile Alcohols, Urine
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​​Random Urine​Urine Tube​​10.0 mL​2.0 mL​
Collection Processing

​Use Serol Analysis Lab requisition

No preservative

Specimen Stability Information
Specimen TypeTemperature​Time
​Urine ​ ​​Frozen (preferred)​Long Term
​Ambient​5 Days
​Refrigerated​11 Days
Reference Range Information

​

​Performing Location​Reference Range
​Oregon Health & Science University​

Normal concentration in unaffected individuals, n=20 < 200 ng/mL

Concentration in CTX-affected untreated individuals, n=8 67,322 ± 39,087 ng/mL [20,022-118,411] mean ± SD [range]

 

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Oregon Health & Science University​Batched Testing​2-4 Weeks​Addition of 23S-pentol-d6 method internal standard followed by incubation of urine with β-glucuronidase enzyme. Isotope-dilution quantification performed using positive-mode LC-ESI-MS/MS multiple reaction monitoring of 23S-pentol (m/z 453.3>361.4) and internal standard (m/z 459.3>343.5) with a reversed-phase gradient utilizing a 4.6x50mm Biphenyl (2.6 µm) column. Linear up to 5,000 ng/mL without dilution. No significant interference from common over the counter and prescription medications. Carry over response in blanks is < 1% of the LLOQ for the analyte and < 0.5% for the internal standard.
Reference Lab
Oregon Health & Science University - Physiology & Pharmacology Department (L334)
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​82542​​1​​
For most current information refer to the Marshfield Laboratory online reference manual.