Organism Referred for Identification, Aerobic Bacteria

Test Code: MISC

Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding

Synonyms/Keywords

Organism Referred for Id, Aerobic Bacteria (IDENT), Organism Referred for Identification, Aerobic Bacteria (IDENT), 50012-IDENT

Useful For

Identification of pure isolates of aerobic bacteria

Differentiation of members of the Staphylococcus aureus complex (S aureus, S argenteus, S schweitzeri)

Specimen Requirements

Fasting Required	Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)	Pediatric Minimum Volume (no repeat)
	Varies: Pure culture of organism form source cultured	Agar slant or other appropriate media		Entire specimen

Collection Processing Instructions

Specimen source is required.

Isolate description is required including: Gram stain reaction, morphology, and test performed.

Collection Instructions:

1. Perform isolation of infecting bacteria

2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.

Specimen Stability Information

Specimen Type	Temperature
Varies	Ambient (preferred)
Varies	Refrigerated

Rejection Criteria

Agar Plate	Reject

Interference

Isolates suspected of being select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) should be submitted to client's state health department or the CDC for identification confirmation or exclusion.

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Analytical Time	Methodology/Instrumentation
Mayo Clinic Laboratories	Monday through Sunday	5-10 Days	Dependent on organism submitted, 1 or more of the following methods will be used: conventional biochemical testing, commercial identification strips or panels, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry, and 16S RNA gene sequencing.

Reference Lab

Mayo Clinic Laboratories

Test Information

Organisms are referred to confirm identification or when the identity is unknown. This may provide helpful information regarding the significance of the organism, its role in the disease process, and its possible origin.

Techniques employed may include conventional biochemical analysis, commercial identification strips or panels, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry or sequencing nucleic acid of the 16S ribosomal RNA (rRNA) gene.

Reference Range Information

Performing Location	Reference Range
Mayo Clinic Laboratories	Identification of organism

Interpretation

Genus and species are reported on aerobic bacterial isolates, whenever possible.

Bacillus species will be reported out as "Large spore-forming aerobic gram-positive Bacillus, not Bacillus cereus or Bacillus anthracis," unless species identification is specifically requested on the request form.

Outreach CPTs

CPT	Quantity	Description	Comments
87077	1	Organism Referred for Identification, Aerobic Bacteria
87077	1	Identification Commercial Kit	if appropriate
87077	1	Ident by MALDI-TOF mass spec	if appropriate
87077	1	Bacteria Identification	if appropriate
87153	1	Aerobe Ident by Sequencing	if appropriate
87077	1	Additional Identification Procedure	if appropriate
87147	3	Serologic Agglut Method 1 Ident	if appropriate
87147	1	Serologic Agglut Method 2 Ident	if appropriate
87147	4	Serologic Agglut Method 3 Ident	if appropriate
87147	2-6	Serologic Agglut Method 4 Ident	if appropriate
87077	1	Identification Staphylococcus	if appropriate
87077	1	Identification Streptococcus	if appropriate
87798	1	Identification by PCR	if appropriate

Synonyms/Keywords

Organism Referred for Id, Aerobic Bacteria (IDENT), Organism Referred for Identification, Aerobic Bacteria (IDENT), 50012-IDENT

Ordering Applications

Ordering Application	Description
COM	Organism Referred for Id, Aerobic Bacteria (IDENT)
Cerner	Organism Referred for Identification, Aerobic Bacteria (IDENT)

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.