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26603 Phenytoin, Free, Serum (PNYF)

Phenytoin, Free, Serum (PNYF)
Test Code: PNYFSO
Synonyms/Keywords

Dilantin Free, Serum
Free Dilantin
Free Dilantin, Serum
Free Phenytoin
Dilantin, Total and Free (ORDER PNTFT) ​​

Test Components

​Phenytoin, Free

Useful For

​Monitoring for appropriate therapeutic concentration of free phenytoin: free phenytoin level is the best indicator of adequate therapy in renal failure.

Assessing compliance and toxicity

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)

​Serum
​​Red Top Tube (RTT)

​2 mL
​1 mL
Collection Processing Instructions

Centrifuge and aliquot serum into a plastic vial within 2 hours of collection.

Specimen Stability Information
Specimen TypeTemperatureTime



​​​Serum




​Refrigerated (preferred)
​7 days
​Frozen
​14 days
​Ambient
​7 days
Rejection Criteria

Gross hemolysis

Interference

​No significant cautionary statements

Performing Laboratory Information
Performing LocationDay(s) Test Performed
​Report Available
Methodology/Instrumentation

​Mayo Clinic Laboratories
​Monday through Sunday
​Same day/1 day
​Membrane Separation/Kinetic Interaction Microparticles in Solution (KIMS) 
Test Information

PHENYTOIN IS THE DRUG OF CHOICE TO TREAT AND PREVENT TONIC-CLONIC AND PSYCHOMOTOR SEIZURES. IF PHENYTOIN ALONE WILL NOT PREVENT SEIZURE ACTIVITY, COADMINISTRATION WITH PHENOBARBITAL IS USUALLY EFFECTIVE.

PHENYTOIN IS HIGHLY PROTEIN-BOUND (90%), MOSTLY TO ALBUMIN. TEN PERCENT OF THE PHENYTOIN CIRCULATES IN THE FREE, UNBOUND FORM. FREE PHENYTOIN IS THE ACTIVE FORM OF THE DRUG, AVAILABLE TO CROSS BIOLOGIC MEMBRANES AND BIND TO RECEPTORS. INCREASED FREE PHENYTOIN PRODUCES AN ENHANCED PHARMACOLOGIC EFFECT. AT THE SAME TIME, THE FREE FRACTION IS MORE AVAILABLE TO THE LIVER TO BE METABOLIZED, SO IT IS CLEARED MORE QUICKLY.

CONCURRENT USE OF PHENYTOIN AND VALPROIC ACID (ANOTHER FREQUENTLY USED ANTIEPILEPTIC) MAY RESULT IN ALTERED VALPROIC ACID LEVELS AND/OR ALTERED PHENYTOIN LEVELS. DUE TO THE COMPLEX SITUATION INVOLVING DISPLACEMENT OF PROTEIN-BOUND PHENYTOIN AND INHIBITION OF PHENYTOIN METABOLISM, AS WELL AS THE POTENTIAL FOR DECREASED VALPROIC ACID CONCENTRATIONS, PATIENTS SHOULD BE MONITORED FOR BOTH PHENYTOIN TOXICITY AND THERAPEUTIC EFFICACY. FREE PHENYTOIN LEVELS SHOULD BE MEASURED TO PROVIDE THE MOST ACCURATE ASSESSMENT OF PHENYTOIN ACTIVITY EARLY IN THERAPY. AT STEADY-STATE, FREE PHENYTOIN AND FREE VALPROIC ACID CONCENTRATIONS SHOULD BE NORMALIZED.

IN RENAL FAILURE, THE OPPORTUNITY FOR THE FREE PHENYTOIN FRACTION TO BE CLEARED IS SIGNIFICANTLY REDUCED. THE END RESULT IS THAT BOTH THE TOTAL AND FREE CONCENTRATION OF PHENYTOIN INCREASE, WITH THE FREE CONCENTRATION INCREASING FASTER THAN THE TOTAL. DOSAGE MUST BE REDUCED TO AVOID TOXICITY. ACCORDINGLY, THE FREE PHENYTOIN LEVEL IS THE BEST INDICATOR OF ADEQUATE THERAPY IN RENAL FAILURE.

TOXICITY IS A CONSTANT POSSIBILITY BECAUSE OF THE MANNER IN WHICH PHENYTOIN IS METABOLIZED. SMALL INCREASES IN DOSE CAN LEAD TO VERY LARGE INCREASES IN BLOOD CONCENTRATION, RESULTING IN EARLY SIGNS OF TOXICITY SUCH AS NYSTAGMUS, ATAXIA, AND DYSARTHRIA. SEVERE TOXICITY IS TYPIFIED BY TREMOR, HYPERREFLEXIA, LETHARGY, AND COMA.

Reference Range Information

THERAPEUTIC: 1.0-2.0 MCG/ML

CRITICAL VALUE: > OR =2.5 MCG/ML

Interpretation

Dose should be adjusted to achieve steady-state blood concentration of free phenytoin between 1.0 and 2.0 mcg/mL. The range for percent free phenytoin is 8% to 14%.

Severe toxicity occurs when the free phenytoin concentration is > or =2.5 mcg/mL. However, response and side effects will be individual.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments

​80186
​1
​Drug screen quantitative phenytoin free
Synonyms/Keywords

Dilantin Free, Serum
Free Dilantin
Free Dilantin, Serum
Free Phenytoin
Dilantin, Total and Free (ORDER PNTFT) ​​

Test Components

​Phenytoin, Free

Ordering Applications
Ordering ApplicationDescription

​Cerner
​​Phenytoin, Free, Serum (PNYF)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)

​Serum
​​Red Top Tube (RTT)

​2 mL
​1 mL
Collection Processing

Centrifuge and aliquot serum into a plastic vial within 2 hours of collection.

Specimen Stability Information
Specimen TypeTemperatureTime



​​​Serum




​Refrigerated (preferred)
​7 days
​Frozen
​14 days
​Ambient
​7 days
Rejection Criteria

Gross hemolysis

Interference

​No significant cautionary statements

Useful For

​Monitoring for appropriate therapeutic concentration of free phenytoin: free phenytoin level is the best indicator of adequate therapy in renal failure.

Assessing compliance and toxicity

Test Components

​Phenytoin, Free

Reference Range Information

THERAPEUTIC: 1.0-2.0 MCG/ML

CRITICAL VALUE: > OR =2.5 MCG/ML

Interpretation

Dose should be adjusted to achieve steady-state blood concentration of free phenytoin between 1.0 and 2.0 mcg/mL. The range for percent free phenytoin is 8% to 14%.

Severe toxicity occurs when the free phenytoin concentration is > or =2.5 mcg/mL. However, response and side effects will be individual.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test Performed
​Report Available
Methodology/Instrumentation

​Mayo Clinic Laboratories
​Monday through Sunday
​Same day/1 day
​Membrane Separation/Kinetic Interaction Microparticles in Solution (KIMS) 
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments

​80186
​1
​Drug screen quantitative phenytoin free
For most current information refer to the Marshfield Laboratory online reference manual.