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26616 Enteric Viral Panel by Nucleic Acid Test

Enteric Viral Panel by Nucleic Acid Test
Test Code: EVPNAT
Synonyms/Keywords

Norovirus, Rotavirus, Adenovirus, Sapovirus, Astrovirus, Feces, Nucleic Acid Test, Enteric Pathogen​​​

Test Components

​DNA isolation, multiplex real-time polymerase chain reaction amplification and detection.​

Useful For

​Detection of Norovirus GI and GII, Rotavirus A, Adenovirus F40/41, Sapovirus (Genogroups I, II, IV, V), Human Astrovirus (hAstro) in patients with gastroenteritis.

Note: Acute diarrhea occurring after 3 days of hospitalization is unlikely to be due to bacterial enteropathogens. Consider a Clostridium difficile toxin nucleic acid assay for C. difficle-associated nosocomial diarrhea instead of stool nucleic acid test. (see Clostridium difficile Toxin Nucleic Acid).

Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
No​
​Feces
​Leak-proof container
​Cary Blair ParaPak C&S
​1 gram
​1 gram
​0.5 gram
Collection Processing Instructions

​​The preferred specimen is fresh stool in a leak-proof container. Transfer approximately 1 gram of stool specimen to a dry, clean container. Avoid contamination with water or urine. ​

If submitting in Cary-Blair, remove cap and place approximately 1 gram of raw stool into the transport medium or a sufficient amount to bring the liquid level up to the “fill to here" line.  

Acceptable Specimen Types

​Feces, Liquid or Soft Raw Stool

Specimen Stability Information
Specimen TypeTemperatureTime
Feces (Preferred)​​
Ambient
​48 hours
​Refrigerated
​120 hours
ParaPak C&S (Cary Blair) filled to the line​
​Ambient
​48 hours
​Refrigerated
​120 hours
Rejection Criteria

​Frozen feces
​Rectal swabs
​Feces outside of stability ranges
​Specimens collected from inpatients after 3 days of hospitalization without laboratory approval
​Duplicate specimens within 24 hours​
​If test is ordered in conjunction with the Enteric Parasite Panel and the Enteric Bacterial Pathogen Panel, all tests will be canceled and the Extended Gastrointestinal Panel will be ordered instead. 

Interference

​Any inhibition of amplification will be detected through the use of an internal amplification control. 

Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Marshfield
​Monday through Friday
​6-8 hours*
​Nucleic Acid Test
*Testing performed once/day.
Sample must be received in the lab by 0600 to be tested same day. ​​​​​ ​
Reference Range Information
Performing LocationReference Range
​Marshfield
​Negative for Norovirus, Rotavirus, Adenovirus, Sapovirus, and Astrovirus

Interpretation

​Negative results in individuals with gastroenteritis may be due to infection by pathogens that are not detected by this test or are non-infectious cases. Positive results do not rule out coinfection with other organisms that may also cause gastroenteritis.

Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​87505
​​​​
Synonyms/Keywords

Norovirus, Rotavirus, Adenovirus, Sapovirus, Astrovirus, Feces, Nucleic Acid Test, Enteric Pathogen​​​

Test Components

​DNA isolation, multiplex real-time polymerase chain reaction amplification and detection.​

Ordering Applications
Ordering ApplicationDescription
​Cerner​
​Enteric Viral Panel by Nucleic Acid Test​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
No​
​Feces
​Leak-proof container
​Cary Blair ParaPak C&S
​1 gram
​1 gram
​0.5 gram
Collection Processing

​​The preferred specimen is fresh stool in a leak-proof container. Transfer approximately 1 gram of stool specimen to a dry, clean container. Avoid contamination with water or urine. ​

If submitting in Cary-Blair, remove cap and place approximately 1 gram of raw stool into the transport medium or a sufficient amount to bring the liquid level up to the “fill to here" line.  

Acceptable Specimen Types

​Feces, Liquid or Soft Raw Stool

Specimen Stability Information
Specimen TypeTemperatureTime
Feces (Preferred)​​
Ambient
​48 hours
​Refrigerated
​120 hours
ParaPak C&S (Cary Blair) filled to the line​
​Ambient
​48 hours
​Refrigerated
​120 hours
Rejection Criteria

​Frozen feces
​Rectal swabs
​Feces outside of stability ranges
​Specimens collected from inpatients after 3 days of hospitalization without laboratory approval
​Duplicate specimens within 24 hours​
​If test is ordered in conjunction with the Enteric Parasite Panel and the Enteric Bacterial Pathogen Panel, all tests will be canceled and the Extended Gastrointestinal Panel will be ordered instead. 

Interference

​Any inhibition of amplification will be detected through the use of an internal amplification control. 

Useful For

​Detection of Norovirus GI and GII, Rotavirus A, Adenovirus F40/41, Sapovirus (Genogroups I, II, IV, V), Human Astrovirus (hAstro) in patients with gastroenteritis.

Note: Acute diarrhea occurring after 3 days of hospitalization is unlikely to be due to bacterial enteropathogens. Consider a Clostridium difficile toxin nucleic acid assay for C. difficle-associated nosocomial diarrhea instead of stool nucleic acid test. (see Clostridium difficile Toxin Nucleic Acid).

Test Components

​DNA isolation, multiplex real-time polymerase chain reaction amplification and detection.​

Reference Range Information
Performing LocationReference Range
​Marshfield
​Negative for Norovirus, Rotavirus, Adenovirus, Sapovirus, and Astrovirus

Interpretation

​Negative results in individuals with gastroenteritis may be due to infection by pathogens that are not detected by this test or are non-infectious cases. Positive results do not rule out coinfection with other organisms that may also cause gastroenteritis.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Marshfield
​Monday through Friday
​6-8 hours*
​Nucleic Acid Test
*Testing performed once/day.
Sample must be received in the lab by 0600 to be tested same day. ​​​​​ ​
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​87505
​​​​
For most current information refer to the Marshfield Laboratory online reference manual.