Cytomegalovirus (CMV) Molecular Detection, PCR, Lower Respiratory (CMVLR)

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Test Code: CMVLRSO

Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding

Synonyms/Keywords

CMV Detection by Real-Time PCR, Cytomegalovirus PCR, CMVLR, Lower respiratory CMV

Test Components

CMVLR, PCR

Useful For

Rapid qualitative detection of cytomegalovirus (CMV) DNA in lower respiratory specimens

This test is not intended for the monitoring of CMV disease progression or response to therapy.

Specimen Requirements

Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)	Pediatric Minimum Volume (no repeat)
Lower Respiratory: Bronchial washing, bronchoalveolar lavage, fluid/washings from lung, sputum, tracheal secretions, tracheal aspirates	Sterile, screwcap, 5-mL aliquot tube	Sterile container	1 mL	0.5 mL

Collection Processing Instructions

Specimen source is required.

Do not centrifuge.

Specimen Stability Information

Specimen Type	Temperature	Time
Varies	Refrigerated (preferred)	7 days
Varies	Frozen	7 days

Rejection Criteria

Lower respiratory swab, Calcium alginate-tipped swab, Wood swab, Transport swab containing gel, Feces, Paraffin blocks, Tissue specimens, Tissue biopsy, Bronchial brushings, Heat-inactivated specimens, Lower respiratory in transport media.

Interference

This test is not validated for lung tissue or biopsy specimens; it is only validated for the lower respiratory specimens indicated in Specimen Required.

Negative results do not preclude cytomegalovirus (CMV) infection and should not be used as the sole basis for treatment or other patient management decisions.

False-negative results may occur if the viral nucleic acid is present at a level below the analytical sensitivity of the assay, if the virus has genomic mutations, insertions, deletions, or rearrangements, or if the assay is performed very early in the course of illness.

The performance of this test has not been established for monitoring treatment of CMV infection.

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Report Available	Methodology/Instrumentation
Mayo Clinic Laboratories	Monday through Sunday	3 to 5 days	Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reference Lab

Mayo Clinic Laboratories

Test Information

Cytomegalovirus (CMV) is a double-stranded DNA virus of the Herpesviridae family. CMV is transmitted through infected body fluids, as well as through sexual contact, organ transplantation, and intrauterine transmission during pregnancy. CMV infection may be asymptomatic but can cause a wide range of symptoms in immunocompromised individuals. Detection of CMV DNA in lower respiratory specimens may support the clinical diagnosis of CMV pneumonitis. Infection with CMV is a significant cause of morbidity and mortality in transplant recipients and other immunocompromised hosts.

Reference Range Information

Negative

Reference values apply to all ages.

Interpretation

A positive result indicates the presence of cytomegalovirus (CMV) DNA in the patient specimen.

A negative result indicates the absence of CMV DNA in the patient specimen but does not rule out possible infection with CMV.

An invalid result indicates the inability to conclusively determine presence or absence of CMV DNA in the patient specimen.

Outreach CPTs

CPT	Modifier (if needed)	Quantity	Description	Comments
87496		1

Synonyms/Keywords

CMV Detection by Real-Time PCR, Cytomegalovirus PCR, CMVLR, Lower respiratory CMV

Test Components

CMVLR, PCR

Ordering Applications

Ordering Application	Description
Cerner	Cytomegalovirus (CMV) Molecular Detection, PCR, Lower Respiratory (CMVLR)

If the ordering application you are looking for is not listed, contact your local laboratory for assistance.

Specimen Requirements

Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)	Pediatric Minimum Volume (no repeat)
Lower Respiratory: Bronchial washing, bronchoalveolar lavage, fluid/washings from lung, sputum, tracheal secretions, tracheal aspirates	Sterile, screwcap, 5-mL aliquot tube	Sterile container	1 mL	0.5 mL

Collection Processing

Specimen source is required.

Do not centrifuge.

Specimen Stability Information

Specimen Type	Temperature	Time
Varies	Refrigerated (preferred)	7 days
Varies	Frozen	7 days

Rejection Criteria

Interference

This test is not validated for lung tissue or biopsy specimens; it is only validated for the lower respiratory specimens indicated in Specimen Required.

Negative results do not preclude cytomegalovirus (CMV) infection and should not be used as the sole basis for treatment or other patient management decisions.

The performance of this test has not been established for monitoring treatment of CMV infection.

Useful For

Rapid qualitative detection of cytomegalovirus (CMV) DNA in lower respiratory specimens

This test is not intended for the monitoring of CMV disease progression or response to therapy.

Test Components

CMVLR, PCR

Reference Range Information

Negative

Reference values apply to all ages.

Interpretation

A positive result indicates the presence of cytomegalovirus (CMV) DNA in the patient specimen.

A negative result indicates the absence of CMV DNA in the patient specimen but does not rule out possible infection with CMV.

An invalid result indicates the inability to conclusively determine presence or absence of CMV DNA in the patient specimen.

For more information visit:

http://labtestsonline.org

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Report Available	Methodology/Instrumentation
Mayo Clinic Laboratories	Monday through Sunday	3 to 5 days	Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reference Lab

Mayo Clinic Laboratories

For billing questions, see Contacts

Outreach CPTs

CPT	Modifier (if needed)	Quantity	Description	Comments
87496		1

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For most current information refer to the Marshfield Laboratory online reference manual.

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26647 Cytomegalovirus (CMV) Molecular Detection, PCR, Lower Respiratory (CMVLR)